bio rad zoe fluorescent cell imager (Bio-Rad)
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Bio Rad Zoe Fluorescent Cell Imager, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1431 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/zoe+fluorescent+cell+imaging+system/ZOE+Fluorescent+Cell+Imager/pm42121903-102-18-18
Average 96 stars, based on 1431 article reviews
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Imaging:Article Title: Green tea extract reduces viral proliferation and ROS production during Feline Herpesvirus type-1 (FHV-1) infection Article Snippet: Cell nuclei were stained with 4′,6-diamidin-2-fenilindolo (DAPI; Vector Laboratories). .. Article Title: Green tea extract reduces viral proliferation and ROS production during Feline Herpesvirus type-1 (FHV-1) infection Article Snippet: .. After treatments, cell monolayers were washed with PBS (1X), fixed, and incubated with a mix of Acridine orange hemi (zinc chloride) salt (Sigma) and PI (Thermo Fisher) for 15 min. After 3 washes with PBS (1X), images were acquired by Article Title: Stiff extracellular matrix activates the transcription factor ATF5 to promote proliferation of cancer cells Article Snippet: Virus-transfected cells were selected by culturing with 4 puromycin (2 g/mL, P9620, Sigma-Aldrich). .. The expression of fluorescent proteins in the transgenic 5 cells was confirmed using the Article Title: Stiff extracellular matrix activates the transcription factor ATF5 to promote the proliferation of cancer cells Article Snippet: Virus-transfected cells were selected by culturing with puromycin (2 μg/mL, P9620, Sigma-Aldrich). .. The expression of fluorescent proteins in the transgenic cells was confirmed using the Article Title: GRK5 is required for adipocyte differentiation through ERK activation Article Snippet: Briefly, cells were seeded into a 6-well plate with a density of 0.1 × 10 6 per well in Dulbecco’s Modified Eagle Medium (DMEM, Gibco, Billings, MT, USA) supplemented with 10% iron-fortified calf serum (CS, Sigma-Aldrich) and 1% penicillin/streptomycin (P/S, Gibco) for 24 h. Cells were then treated with EdU (5-ethynyl-2’-deoxyuridine) solution and incubated for 24 h. EdU, a nucleoside analog of thymidine, was incorporated into newly synthesized DNA and fluorescently labeled with a bright, photostable Alexa FluorTM 647 dye. .. Total DNA was stained using Hoechst 3342 and imaged using BioRad Article Title: Hepatitis C Virus Dysregulates Polyamine and Proline Metabolism and Perturbs the Urea Cycle Article Snippet: Huh7.5 cells were seeded on 24-well plates, infected as described above, fixed with methanol–acetone mixture, and stained with rabbit sera raised to HCV NS3 and secondary FITC-labelled antibodies to rabbit immunoglobulins (F0382, Sigma). .. The cells were visualized by confocal microscopy as reported previously in [ ] or using a Article Title: Non-Lethal Concentrations of CdCl 2 Cause Marked Alternations in Cellular Stress Responses within Exposed Sertoli Cell Line Article Snippet: Then, the cells were incubated at 37 °C for 20 min. After triple washes with serum-free medium to remove free DCFH-DA completely, the cells were subjected to incubation under indicated experimental conditions for 2 h. The fluorescence intensity was monitored at 480 nm with a ZOE Fluorescent Cell Imaging System (Bio-rad, Hercules, CA, USA). .. At the end of the incubation period (12 h), the cells were washed with PBS followed by a fixation with 4% PFA for 20 min and permeabilization with 0.3% Triton X-100 (PBS) at room temperature for 5 min. Then, the cells were incubated with a combination of TdT enzyme and fluorescent labeling solution (Beyotime Institute of Biotechnology, Haimen, China) at 37 °C for 60 min. After triple washes with PBS, the fluorescence intensity was monitored with the Incubation:Article Title: Green tea extract reduces viral proliferation and ROS production during Feline Herpesvirus type-1 (FHV-1) infection Article Snippet: .. After treatments, cell monolayers were washed with PBS (1X), fixed, and incubated with a mix of Acridine orange hemi (zinc chloride) salt (Sigma) and PI (Thermo Fisher) for 15 min. After 3 washes with PBS (1X), images were acquired by Article Title: Non-Lethal Concentrations of CdCl 2 Cause Marked Alternations in Cellular Stress Responses within Exposed Sertoli Cell Line Article Snippet: Then, the cells were incubated at 37 °C for 20 min. After triple washes with serum-free medium to remove free DCFH-DA completely, the cells were subjected to incubation under indicated experimental conditions for 2 h. The fluorescence intensity was monitored at 480 nm with a ZOE Fluorescent Cell Imaging System (Bio-rad, Hercules, CA, USA). .. At the end of the incubation period (12 h), the cells were washed with PBS followed by a fixation with 4% PFA for 20 min and permeabilization with 0.3% Triton X-100 (PBS) at room temperature for 5 min. Then, the cells were incubated with a combination of TdT enzyme and fluorescent labeling solution (Beyotime Institute of Biotechnology, Haimen, China) at 37 °C for 60 min. After triple washes with PBS, the fluorescence intensity was monitored with the Expressing:Article Title: Stiff extracellular matrix activates the transcription factor ATF5 to promote proliferation of cancer cells Article Snippet: Virus-transfected cells were selected by culturing with 4 puromycin (2 g/mL, P9620, Sigma-Aldrich). .. The expression of fluorescent proteins in the transgenic 5 cells was confirmed using the Article Title: Stiff extracellular matrix activates the transcription factor ATF5 to promote the proliferation of cancer cells Article Snippet: Virus-transfected cells were selected by culturing with puromycin (2 μg/mL, P9620, Sigma-Aldrich). .. The expression of fluorescent proteins in the transgenic cells was confirmed using the Transgenic Assay:Article Title: Stiff extracellular matrix activates the transcription factor ATF5 to promote proliferation of cancer cells Article Snippet: Virus-transfected cells were selected by culturing with 4 puromycin (2 g/mL, P9620, Sigma-Aldrich). .. The expression of fluorescent proteins in the transgenic 5 cells was confirmed using the Article Title: Stiff extracellular matrix activates the transcription factor ATF5 to promote the proliferation of cancer cells Article Snippet: Virus-transfected cells were selected by culturing with puromycin (2 μg/mL, P9620, Sigma-Aldrich). .. The expression of fluorescent proteins in the transgenic cells was confirmed using the Staining:Article Title: GRK5 is required for adipocyte differentiation through ERK activation Article Snippet: Briefly, cells were seeded into a 6-well plate with a density of 0.1 × 10 6 per well in Dulbecco’s Modified Eagle Medium (DMEM, Gibco, Billings, MT, USA) supplemented with 10% iron-fortified calf serum (CS, Sigma-Aldrich) and 1% penicillin/streptomycin (P/S, Gibco) for 24 h. Cells were then treated with EdU (5-ethynyl-2’-deoxyuridine) solution and incubated for 24 h. EdU, a nucleoside analog of thymidine, was incorporated into newly synthesized DNA and fluorescently labeled with a bright, photostable Alexa FluorTM 647 dye. .. Total DNA was stained using Hoechst 3342 and imaged using BioRad Confocal Microscopy:Article Title: Hepatitis C Virus Dysregulates Polyamine and Proline Metabolism and Perturbs the Urea Cycle Article Snippet: Huh7.5 cells were seeded on 24-well plates, infected as described above, fixed with methanol–acetone mixture, and stained with rabbit sera raised to HCV NS3 and secondary FITC-labelled antibodies to rabbit immunoglobulins (F0382, Sigma). .. The cells were visualized by confocal microscopy as reported previously in [ ] or using a Labeling:Article Title: Non-Lethal Concentrations of CdCl 2 Cause Marked Alternations in Cellular Stress Responses within Exposed Sertoli Cell Line Article Snippet: Then, the cells were incubated at 37 °C for 20 min. After triple washes with serum-free medium to remove free DCFH-DA completely, the cells were subjected to incubation under indicated experimental conditions for 2 h. The fluorescence intensity was monitored at 480 nm with a ZOE Fluorescent Cell Imaging System (Bio-rad, Hercules, CA, USA). .. At the end of the incubation period (12 h), the cells were washed with PBS followed by a fixation with 4% PFA for 20 min and permeabilization with 0.3% Triton X-100 (PBS) at room temperature for 5 min. Then, the cells were incubated with a combination of TdT enzyme and fluorescent labeling solution (Beyotime Institute of Biotechnology, Haimen, China) at 37 °C for 60 min. After triple washes with PBS, the fluorescence intensity was monitored with the Fluorescence:Article Title: Non-Lethal Concentrations of CdCl 2 Cause Marked Alternations in Cellular Stress Responses within Exposed Sertoli Cell Line Article Snippet: Then, the cells were incubated at 37 °C for 20 min. After triple washes with serum-free medium to remove free DCFH-DA completely, the cells were subjected to incubation under indicated experimental conditions for 2 h. The fluorescence intensity was monitored at 480 nm with a ZOE Fluorescent Cell Imaging System (Bio-rad, Hercules, CA, USA). .. At the end of the incubation period (12 h), the cells were washed with PBS followed by a fixation with 4% PFA for 20 min and permeabilization with 0.3% Triton X-100 (PBS) at room temperature for 5 min. Then, the cells were incubated with a combination of TdT enzyme and fluorescent labeling solution (Beyotime Institute of Biotechnology, Haimen, China) at 37 °C for 60 min. After triple washes with PBS, the fluorescence intensity was monitored with the |
