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bio rad zoe fluorescent cell imager  (Bio-Rad)


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    Bio-Rad bio rad zoe fluorescent cell imager
    Bio Rad Zoe Fluorescent Cell Imager, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1431 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/zoe+fluorescent+cell+imaging+system/ZOE+Fluorescent+Cell+Imager/pm42121903-102-18-18
    Average 96 stars, based on 1431 article reviews
    bio rad zoe fluorescent cell imager - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Imaging:

    Article Title: Green tea extract reduces viral proliferation and ROS production during Feline Herpesvirus type-1 (FHV-1) infection
    Article Snippet: Cell nuclei were stained with 4′,6-diamidin-2-fenilindolo (DAPI; Vector Laboratories). .. ZOE Fluorescent Cell Imaging System (Bio-Rad) was used for images acquisition. ..

    Article Title: Green tea extract reduces viral proliferation and ROS production during Feline Herpesvirus type-1 (FHV-1) infection
    Article Snippet: .. After treatments, cell monolayers were washed with PBS (1X), fixed, and incubated with a mix of Acridine orange hemi (zinc chloride) salt (Sigma) and PI (Thermo Fisher) for 15 min. After 3 washes with PBS (1X), images were acquired by ZOE Fluorescent Cell Imaging System (Bio-Rad). ..

    Article Title: Stiff extracellular matrix activates the transcription factor ATF5 to promote proliferation of cancer cells
    Article Snippet: Virus-transfected cells were selected by culturing with 4 puromycin (2 g/mL, P9620, Sigma-Aldrich). .. The expression of fluorescent proteins in the transgenic 5 cells was confirmed using the ZOE Fluorescent Cell Imaging System (BioRad). .. 6 The following inhibitors and antibodies were used for cell culture: Dimethyl Sulfoxide (DMSO, 7 1:1,000, 046-21981, Wako), JAK inhibitor I (5 M, 420099, Sigma-Aldrich), MYCi361 (5 or 10 M, 8 T12132, TargetMol), Latrunculin A (0.1 M, 125-04363, Wako), Blebbistatin (17 M, BML-EI315-9 0005, Enzo Life Sciences), control rat IgG (2 g/mL, I8015, Sigma-Aldrich or 147-09521, Wako), 10 AIIB2 (2 g/mL, Developmental Studies Hybridoma Bank at the University of Iowa), Y27632 (10 11 M, Y0503, Sigma-Aldrich), PF-562271 (10 M, PZ0387, Sigma-Aldrich), and Calyculin A (2 nM, 12 208851, Sigma-Aldrich).

    Article Title: Stiff extracellular matrix activates the transcription factor ATF5 to promote the proliferation of cancer cells
    Article Snippet: Virus-transfected cells were selected by culturing with puromycin (2 μg/mL, P9620, Sigma-Aldrich). .. The expression of fluorescent proteins in the transgenic cells was confirmed using the ZOE Fluorescent Cell Imaging System (BioRad). .. The following inhibitors and antibodies were used for cell culture: Dimethyl Sulfoxide (DMSO, 1:1,000, 046–21981, Wako), JAK inhibitor I (5 μM, 420099, Sigma-Aldrich), MYCi361 (5 or 10 μM, T12132, TargetMol), Latrunculin A (0.1 μM, 125–04363, Wako), Blebbistatin (17 μM, BML-EI315-0005, Enzo Life Sciences), control rat IgG (2 μg/mL, I8015, Sigma-Aldrich or 147–09521, Wako), AIIB2 (2 μg/mL, Developmental Studies Hybridoma Bank at the University of Iowa), Y27632 (10 μM, Y0503, Sigma-Aldrich), PF-562271 (10 μM, PZ0387, Sigma-Aldrich), and Calyculin A (2 nM, 208851, Sigma-Aldrich).

    Article Title: GRK5 is required for adipocyte differentiation through ERK activation
    Article Snippet: Briefly, cells were seeded into a 6-well plate with a density of 0.1 × 10 6 per well in Dulbecco’s Modified Eagle Medium (DMEM, Gibco, Billings, MT, USA) supplemented with 10% iron-fortified calf serum (CS, Sigma-Aldrich) and 1% penicillin/streptomycin (P/S, Gibco) for 24 h. Cells were then treated with EdU (5-ethynyl-2’-deoxyuridine) solution and incubated for 24 h. EdU, a nucleoside analog of thymidine, was incorporated into newly synthesized DNA and fluorescently labeled with a bright, photostable Alexa FluorTM 647 dye. .. Total DNA was stained using Hoechst 3342 and imaged using BioRad ZOE Fluorescent Cell Imaging System. ..

    Article Title: Hepatitis C Virus Dysregulates Polyamine and Proline Metabolism and Perturbs the Urea Cycle
    Article Snippet: Huh7.5 cells were seeded on 24-well plates, infected as described above, fixed with methanol–acetone mixture, and stained with rabbit sera raised to HCV NS3 and secondary FITC-labelled antibodies to rabbit immunoglobulins (F0382, Sigma). .. The cells were visualized by confocal microscopy as reported previously in [ ] or using a Zoe fluorescent cell imaging system (Bio-Rad, Hercules, CA, USA). ..

    Article Title: Non-Lethal Concentrations of CdCl 2 Cause Marked Alternations in Cellular Stress Responses within Exposed Sertoli Cell Line
    Article Snippet: Then, the cells were incubated at 37 °C for 20 min. After triple washes with serum-free medium to remove free DCFH-DA completely, the cells were subjected to incubation under indicated experimental conditions for 2 h. The fluorescence intensity was monitored at 480 nm with a ZOE Fluorescent Cell Imaging System (Bio-rad, Hercules, CA, USA). .. At the end of the incubation period (12 h), the cells were washed with PBS followed by a fixation with 4% PFA for 20 min and permeabilization with 0.3% Triton X-100 (PBS) at room temperature for 5 min. Then, the cells were incubated with a combination of TdT enzyme and fluorescent labeling solution (Beyotime Institute of Biotechnology, Haimen, China) at 37 °C for 60 min. After triple washes with PBS, the fluorescence intensity was monitored with the ZOE Fluorescent Cell Imaging System (Bio-rad, Hercules, CA, USA) at 480 nm. ..

    Incubation:

    Article Title: Green tea extract reduces viral proliferation and ROS production during Feline Herpesvirus type-1 (FHV-1) infection
    Article Snippet: .. After treatments, cell monolayers were washed with PBS (1X), fixed, and incubated with a mix of Acridine orange hemi (zinc chloride) salt (Sigma) and PI (Thermo Fisher) for 15 min. After 3 washes with PBS (1X), images were acquired by ZOE Fluorescent Cell Imaging System (Bio-Rad). ..

    Article Title: Non-Lethal Concentrations of CdCl 2 Cause Marked Alternations in Cellular Stress Responses within Exposed Sertoli Cell Line
    Article Snippet: Then, the cells were incubated at 37 °C for 20 min. After triple washes with serum-free medium to remove free DCFH-DA completely, the cells were subjected to incubation under indicated experimental conditions for 2 h. The fluorescence intensity was monitored at 480 nm with a ZOE Fluorescent Cell Imaging System (Bio-rad, Hercules, CA, USA). .. At the end of the incubation period (12 h), the cells were washed with PBS followed by a fixation with 4% PFA for 20 min and permeabilization with 0.3% Triton X-100 (PBS) at room temperature for 5 min. Then, the cells were incubated with a combination of TdT enzyme and fluorescent labeling solution (Beyotime Institute of Biotechnology, Haimen, China) at 37 °C for 60 min. After triple washes with PBS, the fluorescence intensity was monitored with the ZOE Fluorescent Cell Imaging System (Bio-rad, Hercules, CA, USA) at 480 nm. ..

    Expressing:

    Article Title: Stiff extracellular matrix activates the transcription factor ATF5 to promote proliferation of cancer cells
    Article Snippet: Virus-transfected cells were selected by culturing with 4 puromycin (2 g/mL, P9620, Sigma-Aldrich). .. The expression of fluorescent proteins in the transgenic 5 cells was confirmed using the ZOE Fluorescent Cell Imaging System (BioRad). .. 6 The following inhibitors and antibodies were used for cell culture: Dimethyl Sulfoxide (DMSO, 7 1:1,000, 046-21981, Wako), JAK inhibitor I (5 M, 420099, Sigma-Aldrich), MYCi361 (5 or 10 M, 8 T12132, TargetMol), Latrunculin A (0.1 M, 125-04363, Wako), Blebbistatin (17 M, BML-EI315-9 0005, Enzo Life Sciences), control rat IgG (2 g/mL, I8015, Sigma-Aldrich or 147-09521, Wako), 10 AIIB2 (2 g/mL, Developmental Studies Hybridoma Bank at the University of Iowa), Y27632 (10 11 M, Y0503, Sigma-Aldrich), PF-562271 (10 M, PZ0387, Sigma-Aldrich), and Calyculin A (2 nM, 12 208851, Sigma-Aldrich).

    Article Title: Stiff extracellular matrix activates the transcription factor ATF5 to promote the proliferation of cancer cells
    Article Snippet: Virus-transfected cells were selected by culturing with puromycin (2 μg/mL, P9620, Sigma-Aldrich). .. The expression of fluorescent proteins in the transgenic cells was confirmed using the ZOE Fluorescent Cell Imaging System (BioRad). .. The following inhibitors and antibodies were used for cell culture: Dimethyl Sulfoxide (DMSO, 1:1,000, 046–21981, Wako), JAK inhibitor I (5 μM, 420099, Sigma-Aldrich), MYCi361 (5 or 10 μM, T12132, TargetMol), Latrunculin A (0.1 μM, 125–04363, Wako), Blebbistatin (17 μM, BML-EI315-0005, Enzo Life Sciences), control rat IgG (2 μg/mL, I8015, Sigma-Aldrich or 147–09521, Wako), AIIB2 (2 μg/mL, Developmental Studies Hybridoma Bank at the University of Iowa), Y27632 (10 μM, Y0503, Sigma-Aldrich), PF-562271 (10 μM, PZ0387, Sigma-Aldrich), and Calyculin A (2 nM, 208851, Sigma-Aldrich).

    Transgenic Assay:

    Article Title: Stiff extracellular matrix activates the transcription factor ATF5 to promote proliferation of cancer cells
    Article Snippet: Virus-transfected cells were selected by culturing with 4 puromycin (2 g/mL, P9620, Sigma-Aldrich). .. The expression of fluorescent proteins in the transgenic 5 cells was confirmed using the ZOE Fluorescent Cell Imaging System (BioRad). .. 6 The following inhibitors and antibodies were used for cell culture: Dimethyl Sulfoxide (DMSO, 7 1:1,000, 046-21981, Wako), JAK inhibitor I (5 M, 420099, Sigma-Aldrich), MYCi361 (5 or 10 M, 8 T12132, TargetMol), Latrunculin A (0.1 M, 125-04363, Wako), Blebbistatin (17 M, BML-EI315-9 0005, Enzo Life Sciences), control rat IgG (2 g/mL, I8015, Sigma-Aldrich or 147-09521, Wako), 10 AIIB2 (2 g/mL, Developmental Studies Hybridoma Bank at the University of Iowa), Y27632 (10 11 M, Y0503, Sigma-Aldrich), PF-562271 (10 M, PZ0387, Sigma-Aldrich), and Calyculin A (2 nM, 12 208851, Sigma-Aldrich).

    Article Title: Stiff extracellular matrix activates the transcription factor ATF5 to promote the proliferation of cancer cells
    Article Snippet: Virus-transfected cells were selected by culturing with puromycin (2 μg/mL, P9620, Sigma-Aldrich). .. The expression of fluorescent proteins in the transgenic cells was confirmed using the ZOE Fluorescent Cell Imaging System (BioRad). .. The following inhibitors and antibodies were used for cell culture: Dimethyl Sulfoxide (DMSO, 1:1,000, 046–21981, Wako), JAK inhibitor I (5 μM, 420099, Sigma-Aldrich), MYCi361 (5 or 10 μM, T12132, TargetMol), Latrunculin A (0.1 μM, 125–04363, Wako), Blebbistatin (17 μM, BML-EI315-0005, Enzo Life Sciences), control rat IgG (2 μg/mL, I8015, Sigma-Aldrich or 147–09521, Wako), AIIB2 (2 μg/mL, Developmental Studies Hybridoma Bank at the University of Iowa), Y27632 (10 μM, Y0503, Sigma-Aldrich), PF-562271 (10 μM, PZ0387, Sigma-Aldrich), and Calyculin A (2 nM, 208851, Sigma-Aldrich).

    Staining:

    Article Title: GRK5 is required for adipocyte differentiation through ERK activation
    Article Snippet: Briefly, cells were seeded into a 6-well plate with a density of 0.1 × 10 6 per well in Dulbecco’s Modified Eagle Medium (DMEM, Gibco, Billings, MT, USA) supplemented with 10% iron-fortified calf serum (CS, Sigma-Aldrich) and 1% penicillin/streptomycin (P/S, Gibco) for 24 h. Cells were then treated with EdU (5-ethynyl-2’-deoxyuridine) solution and incubated for 24 h. EdU, a nucleoside analog of thymidine, was incorporated into newly synthesized DNA and fluorescently labeled with a bright, photostable Alexa FluorTM 647 dye. .. Total DNA was stained using Hoechst 3342 and imaged using BioRad ZOE Fluorescent Cell Imaging System. ..

    Confocal Microscopy:

    Article Title: Hepatitis C Virus Dysregulates Polyamine and Proline Metabolism and Perturbs the Urea Cycle
    Article Snippet: Huh7.5 cells were seeded on 24-well plates, infected as described above, fixed with methanol–acetone mixture, and stained with rabbit sera raised to HCV NS3 and secondary FITC-labelled antibodies to rabbit immunoglobulins (F0382, Sigma). .. The cells were visualized by confocal microscopy as reported previously in [ ] or using a Zoe fluorescent cell imaging system (Bio-Rad, Hercules, CA, USA). ..

    Labeling:

    Article Title: Non-Lethal Concentrations of CdCl 2 Cause Marked Alternations in Cellular Stress Responses within Exposed Sertoli Cell Line
    Article Snippet: Then, the cells were incubated at 37 °C for 20 min. After triple washes with serum-free medium to remove free DCFH-DA completely, the cells were subjected to incubation under indicated experimental conditions for 2 h. The fluorescence intensity was monitored at 480 nm with a ZOE Fluorescent Cell Imaging System (Bio-rad, Hercules, CA, USA). .. At the end of the incubation period (12 h), the cells were washed with PBS followed by a fixation with 4% PFA for 20 min and permeabilization with 0.3% Triton X-100 (PBS) at room temperature for 5 min. Then, the cells were incubated with a combination of TdT enzyme and fluorescent labeling solution (Beyotime Institute of Biotechnology, Haimen, China) at 37 °C for 60 min. After triple washes with PBS, the fluorescence intensity was monitored with the ZOE Fluorescent Cell Imaging System (Bio-rad, Hercules, CA, USA) at 480 nm. ..

    Fluorescence:

    Article Title: Non-Lethal Concentrations of CdCl 2 Cause Marked Alternations in Cellular Stress Responses within Exposed Sertoli Cell Line
    Article Snippet: Then, the cells were incubated at 37 °C for 20 min. After triple washes with serum-free medium to remove free DCFH-DA completely, the cells were subjected to incubation under indicated experimental conditions for 2 h. The fluorescence intensity was monitored at 480 nm with a ZOE Fluorescent Cell Imaging System (Bio-rad, Hercules, CA, USA). .. At the end of the incubation period (12 h), the cells were washed with PBS followed by a fixation with 4% PFA for 20 min and permeabilization with 0.3% Triton X-100 (PBS) at room temperature for 5 min. Then, the cells were incubated with a combination of TdT enzyme and fluorescent labeling solution (Beyotime Institute of Biotechnology, Haimen, China) at 37 °C for 60 min. After triple washes with PBS, the fluorescence intensity was monitored with the ZOE Fluorescent Cell Imaging System (Bio-rad, Hercules, CA, USA) at 480 nm. ..



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    Bio-Rad fluorescent cell imager
    Long-term persistence of viable E. ruminantium within G. mellonella hemocytes. (A) Fluorescence microscopy of hemolymph collected 6 days post-infection, showing sustained intracellular bacterial presence within hemocytes. Nuclei are stained with Hoechst (blue) and bacteria with rhodamine (red). Scale bar, 30 μm. (B) Stereomicroscopy demonstrating whole-larva bacterial fluorescence persistence at 8 days post-infection. Scale bar, 5 mm. (C) Viability confirmation through re-infection assay. Hemolymph from infected G. mellonella was inoculated onto bovine aortic endothelial cell (BAEC) monolayers. At 4 days post-inoculation, characteristic morulae formation, lysis areas, and <t>fluorescent</t> bacteria within BAEC confirm maintenance of bacterial viability and infectivity throughout the larval infection period. Scale bar, 100 μm. N, nuclei; DPI, days post-infection.
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    Image Search Results


    Long-term persistence of viable E. ruminantium within G. mellonella hemocytes. (A) Fluorescence microscopy of hemolymph collected 6 days post-infection, showing sustained intracellular bacterial presence within hemocytes. Nuclei are stained with Hoechst (blue) and bacteria with rhodamine (red). Scale bar, 30 μm. (B) Stereomicroscopy demonstrating whole-larva bacterial fluorescence persistence at 8 days post-infection. Scale bar, 5 mm. (C) Viability confirmation through re-infection assay. Hemolymph from infected G. mellonella was inoculated onto bovine aortic endothelial cell (BAEC) monolayers. At 4 days post-inoculation, characteristic morulae formation, lysis areas, and fluorescent bacteria within BAEC confirm maintenance of bacterial viability and infectivity throughout the larval infection period. Scale bar, 100 μm. N, nuclei; DPI, days post-infection.

    Journal: bioRxiv

    Article Title: Galleria mellonella as a novel invertebrate model for studying Ehrlichia ruminantium pathogenesis and host-pathogen interactions

    doi: 10.64898/2026.04.29.721731

    Figure Lengend Snippet: Long-term persistence of viable E. ruminantium within G. mellonella hemocytes. (A) Fluorescence microscopy of hemolymph collected 6 days post-infection, showing sustained intracellular bacterial presence within hemocytes. Nuclei are stained with Hoechst (blue) and bacteria with rhodamine (red). Scale bar, 30 μm. (B) Stereomicroscopy demonstrating whole-larva bacterial fluorescence persistence at 8 days post-infection. Scale bar, 5 mm. (C) Viability confirmation through re-infection assay. Hemolymph from infected G. mellonella was inoculated onto bovine aortic endothelial cell (BAEC) monolayers. At 4 days post-inoculation, characteristic morulae formation, lysis areas, and fluorescent bacteria within BAEC confirm maintenance of bacterial viability and infectivity throughout the larval infection period. Scale bar, 100 μm. N, nuclei; DPI, days post-infection.

    Article Snippet: Samples were stained with 100 μL of 10 μg/mL Hoechst 33342 (Sigma-Aldrich) for 15-30 minutes at room temperature before microscopic examination using a fluorescent cell imager (ZOE, BioRad).

    Techniques: Fluorescence, Microscopy, Infection, Staining, Bacteria, Lysis